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Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii (Download the PDF)* l. b8 J7 \, G+ @4 k' q& S+ T
Ying-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*
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+ M7 l3 e) c. n5 y/ V/ ^0 MAbstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.* P2 K4 m: B7 J
Therefore, bats may have evolved effective mechanisms to control viral replication. However, little& l/ r# r9 {4 t
information is available on bat immune responses to viral infection. Type I interferon (IFN) plays a
$ e+ w' {- r0 J, [6 ^, zkey role in controlling viral infections. In this study, we report the cloning, expression, and( F# d( W1 n. c& j
biological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We
/ N$ N0 z% `8 N# c8 ~demonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from
, V) y2 r5 l7 O7 EM. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant
* S- w( t& w2 h1 I! m3 WIFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat+ f1 V* z2 j5 x8 \2 i3 J. A
species, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral
" J3 Y- A# F' U3 hactivity in microbats, which has important implications for virus interactions with these hosts.6 _ w, X1 L) Y2 h
% G4 Y% y. @: ]8 y( ]( LKeywords: bat; interferon; IFN-stimulated genes; antiviral activity
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& r6 ]2 c# s6 z4 E+ S; r中文信息：7 h: y% h. X4 b0 H- u+ R" p
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大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测
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% D: Y1 |5 i3 p: x4 g7 x* D6 j梁英子，吴利军，张倩，周鹏，王媚娘，杨兴娄，葛行义，王林发，石正丽*
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. `- }5 m% e- i4 ]( B6 _摘要：蝙蝠是多种病毒的自然宿主，其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状，其中机制未知。近期对两种蝙蝠基因组序列分析结果提示，蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上，用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平；表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白，测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用，试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。
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关键词：干扰素，蝙蝠天然免疫，抗病毒活性，定量PCR" n. }' l& P& u
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发表于 2015-12-7 17:01:33
发表于 2015-12-7 22:28:08
