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Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii (Download the PDF)
: ` T: ]- C8 f& vYing-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*
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' }: `- k, w0 P/ Z5 xAbstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.
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information is available on bat immune responses to viral infection. Type I interferon (IFN) plays a
) q& f- S; A3 [% skey role in controlling viral infections. In this study, we report the cloning, expression, and
( t1 p d# G- ^* lbiological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We
( C+ P/ R/ g- f3 qdemonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from
* L, W$ c) x, m0 x/ z3 eM. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant o3 _/ J; I- b
IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat
. \ S: D. i0 g& tspecies, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral
" N {& G: T6 S: v) L( t2 jactivity in microbats, which has important implications for virus interactions with these hosts. ], T0 N" k/ W* @, O' J
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Keywords: bat; interferon; IFN-stimulated genes; antiviral activity
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中文信息：
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大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测
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% l5 F. ^2 D5 B7 \* k梁英子，吴利军，张倩，周鹏，王媚娘，杨兴娄，葛行义，王林发，石正丽*3 g5 S: Z" w3 o: K& L1 \! {
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9 n. v2 T8 F5 Q1 v" `摘要：蝙蝠是多种病毒的自然宿主，其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状，其中机制未知。近期对两种蝙蝠基因组序列分析结果提示，蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上，用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平；表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白，测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用，试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。+ _4 d: q( q, J5 U* {
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4 ~ s- R( t4 d7 C O: \" ^5 s关键词：干扰素，蝙蝠天然免疫，抗病毒活性，定量PCR
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发表于 2015-12-7 17:01:33
发表于 2015-12-7 22:28:08
