Virologica Sinica 精彩论文抢先看！

lishaojie

Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii           (Download the PDF)
8 @4 O0 _# W) CYing-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*

Abstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.
' g" q8 e8 R6 \; S  @; n1 bTherefore, bats may have evolved effective mechanisms to control viral replication. However, little
$ Z9 M3 w# d9 d) _7 D0 Ainformation is available on bat immune responses to viral infection. Type I interferon (IFN) plays a
  {2 Z3 _( }9 s2 vkey role in controlling viral infections. In this study, we report the cloning, expression, and
/ v/ U4 ?6 N+ xbiological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We
demonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from
M. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant
IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat
2 W* v  o) \) Lspecies, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral
' n4 R0 o1 Y% @& e1 ~activity in microbats, which has important implications for virus interactions with these hosts.
/ W; ?9 g" B- g1 h7 m* b7 t
Keywords: bat; interferon; IFN-stimulated genes; antiviral activity

; `6 {. @7 I# i& Q$ g3 x
- `* j# W0 Y7 j中文信息：

) }- Y' c" S0 B2 ^9 Z. K' R大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测

- A7 c6 W. f- \9 w
梁英子，吴利军，张倩，周鹏，王媚娘，杨兴娄，葛行义，王林发，石正丽*
1 s2 @, ~& i0 z  X( _

摘要：蝙蝠是多种病毒的自然宿主，其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状，其中机制未知。近期对两种蝙蝠基因组序列分析结果提示，蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上，用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平；表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白，测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用，试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。
' g% _& A& c, G) v

4 c! Q( f6 `) Y7 g* k2 V关键词：干扰素，蝙蝠天然免疫，抗病毒活性，定量PCR

0 N! Q' E( D. z; _/ W! W+ \

rojjer

不错！