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Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii (Download the PDF)8 e& P! t4 o/ U! x5 \/ N7 t& D
Ying-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*% E! o& k, J% e: D9 G% v! {) a" F
" r+ _( t& o1 o% fAbstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.
/ d0 s& G% O1 y4 o) C- BTherefore, bats may have evolved effective mechanisms to control viral replication. However, little5 |* B5 ^6 H) o/ g* j- v
information is available on bat immune responses to viral infection. Type I interferon (IFN) plays a
' b. l& T6 O' {9 X& wkey role in controlling viral infections. In this study, we report the cloning, expression, and4 t% O1 x9 p4 G! k5 D' M$ H' i
biological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We
* k% K5 v% C+ y! n8 Y% ^6 Y/ vdemonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from
- S7 M- Z/ k; W4 kM. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant; v( k, C, I- N- F. f+ i
IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat
& @! u7 ^& s3 c% u1 r& {species, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral
1 {5 Y7 O, ]- l8 g8 c V% { F$ B' qactivity in microbats, which has important implications for virus interactions with these hosts.2 F! d# C; M* y; }
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Keywords: bat; interferon; IFN-stimulated genes; antiviral activity
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中文信息：+ S$ A+ Z v2 @6 _# X8 L
* d8 k& v$ ~0 Q* S# }* U大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测! [" f, y6 b8 D8 H; T. ?
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# h' G0 r+ e& V$ o/ c( |$ m梁英子，吴利军，张倩，周鹏，王媚娘，杨兴娄，葛行义，王林发，石正丽*; U( O8 U% ^- h1 U
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摘要：蝙蝠是多种病毒的自然宿主，其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状，其中机制未知。近期对两种蝙蝠基因组序列分析结果提示，蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上，用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平；表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白，测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用，试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。
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' [7 ]' r/ N' J6 _, J关键词：干扰素，蝙蝠天然免疫，抗病毒活性，定量PCR
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发表于 2015-12-7 17:01:33
发表于 2015-12-7 22:28:08
