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Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii (Download the PDF)! G8 u L2 _5 H8 i8 W
Ying-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*
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Abstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.
$ _. c0 ]6 M. W' Q# W6 u* W6 Z% PTherefore, bats may have evolved effective mechanisms to control viral replication. However, little
& k# c$ a# N% b3 z) U: v. uinformation is available on bat immune responses to viral infection. Type I interferon (IFN) plays a7 X: J2 G9 X2 i" q$ z k* W
key role in controlling viral infections. In this study, we report the cloning, expression, and
9 @, M1 _7 d7 \3 X! I3 {& w2 hbiological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We, w( @& o' h- W: ~
demonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from+ J3 }' h" J! s. C6 T2 @0 Q/ t, ^
M. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant) l1 s% C+ h8 x; y4 F7 {4 K& E
IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat( O" X7 Q* t' E
species, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral5 ~! h- j8 t9 |
activity in microbats, which has important implications for virus interactions with these hosts.' d/ u# x$ P5 J' R1 t. L* Y3 I
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Keywords: bat; interferon; IFN-stimulated genes; antiviral activity% u0 g8 k$ p2 E6 k) |5 M0 [" F
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中文信息：
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, Z: i" t, v" G- ^+ z% k大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测
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梁英子，吴利军，张倩，周鹏，王媚娘，杨兴娄，葛行义，王林发，石正丽*
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摘要：蝙蝠是多种病毒的自然宿主，其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状，其中机制未知。近期对两种蝙蝠基因组序列分析结果提示，蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上，用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平；表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白，测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用，试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。5 }* M, E" A5 ?6 \) H5 L+ i6 r& B3 E+ N3 L
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0 a$ k3 b0 K/ O; [ S6 u; X! y7 b5 U( |关键词：干扰素，蝙蝠天然免疫，抗病毒活性，定量PCR) O- F) F( f! A6 h H
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发表于 2015-12-7 17:01:33
发表于 2015-12-7 22:28:08
