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Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii (Download the PDF), j! k- ?% m/ x7 ~
Ying-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*4 @/ @+ ]6 R8 E- N7 S
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Abstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.; q, ~/ M w. o5 @" h
Therefore, bats may have evolved effective mechanisms to control viral replication. However, little9 W6 S3 f( m, B
information is available on bat immune responses to viral infection. Type I interferon (IFN) plays a% C: W) k/ p; v9 d1 K! |" g8 z
key role in controlling viral infections. In this study, we report the cloning, expression, and
( A! Z8 r' d: L. K$ q% Kbiological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We
: k- I F; q: a; b% z/ G- udemonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from; G% _1 Z8 N6 I9 L" Y
M. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant
1 c* u; b' N$ K* ^+ D+ e: K: |IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat
6 R9 m$ ~9 u$ k5 Hspecies, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral' k. t% E6 f7 v8 u$ d
activity in microbats, which has important implications for virus interactions with these hosts.( Y- E# R$ p/ v2 b2 Q0 N1 r0 B* e
: ?% |1 i- T* A) e% f2 mKeywords: bat; interferon; IFN-stimulated genes; antiviral activity
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大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测
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9 t# i. z, B4 ]; P梁英子，吴利军，张倩，周鹏，王媚娘，杨兴娄，葛行义，王林发，石正丽*
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摘要：蝙蝠是多种病毒的自然宿主，其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状，其中机制未知。近期对两种蝙蝠基因组序列分析结果提示，蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上，用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平；表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白，测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用，试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。+ z: r2 E: `! P7 B, O1 \
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关键词：干扰素，蝙蝠天然免疫，抗病毒活性，定量PCR; m* s5 j5 w9 E" G1 s# i2 p, n
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发表于 2015-12-7 17:01:33
发表于 2015-12-7 22:28:08
