禽流感病毒Avian Influenza Virus (Methods in Molecular Biology)

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08年新书 Avian Influenza Virus
【书名版次】：Avian Influenza Virus（First edition）(Methods in Molecular Biology Series 436)  
6 x( N8 B4 g- L7 G& i【作    者】：Erica Spackman Editor
+ Z, k9 |8 N+ {" u0 j【出 版 社】: Humana Press
( ]5 b% Q1 w6 k( Y; N& {8 T' m& z【简    介】：
# L" [5 [9 ~6 E, V6 a+ [2 \* P   08新书,大家共享    附件PDF下载！

1 _. Z! i; A3 i" r# q) @   The aim of this book is to create a resource that includes both basic methods that
( |- v1 e8 g# |8 m# h' c/ L( ~are currently used and well established as well as some of the most exciting new
/ J- n/ G$ s* j: dmethods for studying the virus itself, and to include methods that focus on work
with avian hosts, an area that has been greatly lacking. In that context, this volume
% n. C4 ^1 f1 }5 Y+ cwill be of interest to both diagnosticians and researchers, but it does assume a basic
knowledge of virology and molecular biology.
3 a% d- Z. w, _+ L7 J2 }I would like to thank all the authors for their contributions. I am also thankful to
/ z6 e  ^1 g5 q- @+ Y* D5 ~- XJohn M. Walker, the series editor, for his help in editing and working with me
through this project.
Erica Spackman
Athens, GA, September 2007

【出版日期】：2008
【目    录】：
' z$ y' `1 p7 e7 E) ]9 c1 oPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
& H! q% k$ b8 q3 S8 q1 SContributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
* C9 A& T& {* j" n1 A Brief Introduction to the Avian Infl uenza Virus. . . . . . . . . . . . . . . . 1
7 l. z$ l; O0 ?! P( Q: MErica Spackman
$ b8 Q1 r' i9 u' j- B4 r4 z2 Avian Infl uenza Virus Sample Types, Collection, and Handling . . . . . 7
$ }& A- w* F' @+ g# U5 oMary Lea Killian
$ ?  @8 C5 L/ N9 t' m  @4 @, R3 Avian Infl uenza Virus RNA Extraction from Tissue
and Swab Material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
9 \, @6 Y! w8 c% rErica Spackman and David L. Suarez
3 y3 x( K2 P, y, B4 p4 Type A Infl uenza Virus Detection and Quantitation
* U) g/ j5 E% m2 f/ f6 W0 aby Real-Time RT-PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Erica Spackman and David L. Suarez
- W9 c2 G! t) @2 F5 Detection and Identifi cation of the H5 Hemagglutinin
9 S+ _* Y3 l) Y3 \- \$ T2 @6 oSubtype by Real-Time RT-PCR. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Erica Spackman and David L. Suarez
+ f1 j- ~7 n+ K6 Avian Infl uenza Virus Isolation and Propagation
; p: `4 w( t) H( o8 m" Pin Chicken Eggs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
. s% Z+ x8 J% b: E4 M  gPeter R. Woolcock
7 Hemagglutination Assay for the Avian Infl uenza Virus . . . . . . . . . . . 47
" _) X" `, k" I/ t: r8 xMary Lea Killian
8 Hemagglutination-Inhibition Test for Avian Infl uenza Virus
$ F% X: X: [" ]Subtype Identifi cation and the Detection and Quantitation
of Serum Antibodies to the Avian Infl uenza Virus . . . . . . . . . . . . . . . 53
/ F* v9 E9 ]5 Y) V8 a4 J$ t' W7 l$ u) YJanice C. Pedersen
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% ^, n1 H+ H. e& Y1 S' x9 Neuraminidase-Inhibition Assay for the Identifi cation
of Infl uenza A Virus Neuraminidase Subtype or
5 ~7 i/ l, W# z) g, VNeuraminidase Antibody Specifi city . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Janice C. Pedersen
10 Immunohistochemical Staining for the Detection of the
Avian Infl uenza Virus in Tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
# ]: x' z. m) N. q7 m' oMary J. Pantin-Jackwood
) `0 `! B1 x) v/ O11 Wild Bird Surveillance for the Avian Infl uenza Virus. . . . . . . . . . . . . 85
Justin D. Brown and David E. Stallknecht
" t9 z$ K$ U" s. x12 Reverse Genetics of the Avian Infl uenza Virus. . . . . . . . . . . . . . . . . . . 99
Chang-Won Lee and David L. Suarez
6 P( u4 J8 y$ N! B4 x( [13 Evaluating the Cell-Mediated Immune Response of Avian
Species to Avian Infl uenza Viruses . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
; l* @# ]# ~& r* WDarrell R. Kapczynski
/ v! P6 N) u6 o6 d) V$ y  S14 Measurement of Avian Cytokines with Real-Time RT-PCR
Following Infection with the Avian Infl uenza Virus . . . . . . . . . . . . . . 127
4 {6 M6 t! b$ a+ B4 ADarrell R. Kapczynski and Michael H. Kogut
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
viii Contents
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