禽流感病毒Avian Influenza Virus (Methods in Molecular Biology)

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! ~" X  Z; u7 E# E08年新书 Avian Influenza Virus
【书名版次】：Avian Influenza Virus（First edition）(Methods in Molecular Biology Series 436)  
【作    者】：Erica Spackman Editor
【出 版 社】: Humana Press
【简    介】：
   08新书,大家共享    附件PDF下载！

& N% d+ A' H2 u/ {; O- {  ]" \   The aim of this book is to create a resource that includes both basic methods that
; i0 ~6 S( [" l3 Bare currently used and well established as well as some of the most exciting new
. K& d+ I5 [7 b+ U" Lmethods for studying the virus itself, and to include methods that focus on work
with avian hosts, an area that has been greatly lacking. In that context, this volume
+ o/ i* c8 V# ]7 O6 n7 ^will be of interest to both diagnosticians and researchers, but it does assume a basic
+ o" l. F7 v, X5 a& H6 |knowledge of virology and molecular biology.
I would like to thank all the authors for their contributions. I am also thankful to
( \  i) r6 `- _0 {* IJohn M. Walker, the series editor, for his help in editing and working with me
through this project.
Erica Spackman
, j! M. v3 L) oAthens, GA, September 2007

【出版日期】：2008
【目    录】：
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
9 }0 q- Y4 D4 q2 Y. EContributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
1 A Brief Introduction to the Avian Infl uenza Virus. . . . . . . . . . . . . . . . 1
$ l. o/ a9 y) i. b. JErica Spackman
# B, \3 l  k0 c2 Avian Infl uenza Virus Sample Types, Collection, and Handling . . . . . 7
Mary Lea Killian
8 \* H" r9 \6 X5 U9 m3 Avian Infl uenza Virus RNA Extraction from Tissue
8 k8 E3 v( D3 f) J% f; n6 zand Swab Material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
. ]- Y9 A3 T8 z, r, IErica Spackman and David L. Suarez
4 Type A Infl uenza Virus Detection and Quantitation
9 V: K& ]  C: _* T7 k; S0 Xby Real-Time RT-PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Erica Spackman and David L. Suarez
! G; e3 j. [) e. q6 W' T5 Detection and Identifi cation of the H5 Hemagglutinin
3 g# o1 M% P( i. n% I5 }! [$ QSubtype by Real-Time RT-PCR. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Erica Spackman and David L. Suarez
6 Avian Infl uenza Virus Isolation and Propagation
( D2 g# t' F* e0 b9 Y" Rin Chicken Eggs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
' f/ f$ u* n. _3 I" U+ [; YPeter R. Woolcock
7 k0 K% i6 s, O7 Hemagglutination Assay for the Avian Infl uenza Virus . . . . . . . . . . . 47
Mary Lea Killian
8 Hemagglutination-Inhibition Test for Avian Infl uenza Virus
/ S) \) t+ C7 U$ QSubtype Identifi cation and the Detection and Quantitation
4 v/ c0 |; P9 |$ `of Serum Antibodies to the Avian Infl uenza Virus . . . . . . . . . . . . . . . 53
+ W" d* _# M7 P( h3 K8 J1 Z7 @Janice C. Pedersen
7 \/ q9 [5 u3 x/ R6 W4 _vii
9 Neuraminidase-Inhibition Assay for the Identifi cation
of Infl uenza A Virus Neuraminidase Subtype or
Neuraminidase Antibody Specifi city . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
; d1 ^' G4 B+ j7 t  g! \Janice C. Pedersen
10 Immunohistochemical Staining for the Detection of the
# Q: y7 L# c0 Y2 H& R& tAvian Infl uenza Virus in Tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
/ k: ~! H4 |5 @0 }  V5 AMary J. Pantin-Jackwood
" k8 k, u, U$ d  M# t* |11 Wild Bird Surveillance for the Avian Infl uenza Virus. . . . . . . . . . . . . 85
Justin D. Brown and David E. Stallknecht
1 `' m$ i- [' q" D3 L7 L( J12 Reverse Genetics of the Avian Infl uenza Virus. . . . . . . . . . . . . . . . . . . 99
Chang-Won Lee and David L. Suarez
; y8 n% P, X3 A13 Evaluating the Cell-Mediated Immune Response of Avian
$ o% L8 c) B; C4 |) P/ ]9 PSpecies to Avian Infl uenza Viruses . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Darrell R. Kapczynski
14 Measurement of Avian Cytokines with Real-Time RT-PCR
+ F3 g  o; A- P, fFollowing Infection with the Avian Infl uenza Virus . . . . . . . . . . . . . . 127
5 L$ a: p) W+ T9 N! `Darrell R. Kapczynski and Michael H. Kogut
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
viii Contents
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