禽流感病毒Avian Influenza Virus (Methods in Molecular Biology)

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8 J4 b6 {/ s) P  R, [8 J08年新书 Avian Influenza Virus
& R& ]: ?3 G( A% r, E. n【书名版次】：Avian Influenza Virus（First edition）(Methods in Molecular Biology Series 436)  
【作    者】：Erica Spackman Editor
( ]+ p* b, q1 Q$ q, m【出 版 社】: Humana Press
【简    介】：
   08新书,大家共享    附件PDF下载！

" C  p0 y/ H( q9 ?   The aim of this book is to create a resource that includes both basic methods that
  M1 D" z% i! @+ _" K  }) a) Fare currently used and well established as well as some of the most exciting new
methods for studying the virus itself, and to include methods that focus on work
. y7 N2 G  y) w& v) Q$ ]  h3 Q- Qwith avian hosts, an area that has been greatly lacking. In that context, this volume
will be of interest to both diagnosticians and researchers, but it does assume a basic
) L/ R: a* n' z' d+ _knowledge of virology and molecular biology.
I would like to thank all the authors for their contributions. I am also thankful to
  Y) X- n5 Z. T) @2 H. }John M. Walker, the series editor, for his help in editing and working with me
through this project.
Erica Spackman
7 t8 v: K4 h# }: x  s0 hAthens, GA, September 2007
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' o$ l) v2 M6 I% F$ g4 ^4 w【出版日期】：2008
【目    录】：
$ j2 h$ o: W" NPreface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
9 F+ C% U8 {; O: j. pContributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
8 ]* y6 f  z  D1 A Brief Introduction to the Avian Infl uenza Virus. . . . . . . . . . . . . . . . 1
. D, v! R  H$ B0 @0 a% ^3 PErica Spackman
2 Avian Infl uenza Virus Sample Types, Collection, and Handling . . . . . 7
Mary Lea Killian
+ G. s; Z( T8 {8 ^- Q5 y3 Avian Infl uenza Virus RNA Extraction from Tissue
6 W: V  k( b" M, U' h" D9 Aand Swab Material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Erica Spackman and David L. Suarez
4 Type A Infl uenza Virus Detection and Quantitation
! ?' [* o7 w% a/ S; b( Hby Real-Time RT-PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
9 N7 P  R6 V3 ~8 l) T8 K& ^Erica Spackman and David L. Suarez
  B4 `8 c: Z2 \7 G* W7 E5 Detection and Identifi cation of the H5 Hemagglutinin
" e- S$ b" _$ D1 a6 Y- c+ `Subtype by Real-Time RT-PCR. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Erica Spackman and David L. Suarez
6 Avian Infl uenza Virus Isolation and Propagation
in Chicken Eggs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
5 D/ n9 H% n2 Y, S) jPeter R. Woolcock
7 Hemagglutination Assay for the Avian Infl uenza Virus . . . . . . . . . . . 47
9 S- b1 x8 e( j" {! MMary Lea Killian
8 Hemagglutination-Inhibition Test for Avian Infl uenza Virus
; d) ]* Q1 F# N4 O5 TSubtype Identifi cation and the Detection and Quantitation
# I# E) y- {2 a, q5 g/ Gof Serum Antibodies to the Avian Infl uenza Virus . . . . . . . . . . . . . . . 53
Janice C. Pedersen
vii
9 Neuraminidase-Inhibition Assay for the Identifi cation
* }8 K! ]4 f* W7 x7 ~: Mof Infl uenza A Virus Neuraminidase Subtype or
! z* ~; n  B+ P9 \Neuraminidase Antibody Specifi city . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
3 D- c6 D- ^' ]4 ~1 J$ GJanice C. Pedersen
10 Immunohistochemical Staining for the Detection of the
Avian Infl uenza Virus in Tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Mary J. Pantin-Jackwood
11 Wild Bird Surveillance for the Avian Infl uenza Virus. . . . . . . . . . . . . 85
# J' d5 x2 w! s3 W2 cJustin D. Brown and David E. Stallknecht
  N( P4 A) W& Q. w! S* ~- A12 Reverse Genetics of the Avian Infl uenza Virus. . . . . . . . . . . . . . . . . . . 99
Chang-Won Lee and David L. Suarez
13 Evaluating the Cell-Mediated Immune Response of Avian
/ R! h# Z9 R' {* ~8 q- \Species to Avian Infl uenza Viruses . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
+ ?( i' [0 @  t+ B& j% F2 LDarrell R. Kapczynski
14 Measurement of Avian Cytokines with Real-Time RT-PCR
3 g/ K/ S5 P, Z/ N- ]Following Infection with the Avian Infl uenza Virus . . . . . . . . . . . . . . 127
Darrell R. Kapczynski and Michael H. Kogut
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
& f8 k* w' |" C% i2 I. n+ q0 rviii Contents
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