禽流感病毒Avian Influenza Virus (Methods in Molecular Biology)

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9 s% z' S! o1 f& k08年新书 Avian Influenza Virus
, K6 E, b2 j: k; A1 Z' M【书名版次】：Avian Influenza Virus（First edition）(Methods in Molecular Biology Series 436)  
【作    者】：Erica Spackman Editor
+ t1 [9 y2 ^2 t4 J- F6 j【出 版 社】: Humana Press
【简    介】：
8 a$ ]$ V+ ]& k/ A+ V* i5 l   08新书,大家共享    附件PDF下载！

# s3 q& O! j7 X! @% f   The aim of this book is to create a resource that includes both basic methods that
% x2 H$ k. f9 g! kare currently used and well established as well as some of the most exciting new
methods for studying the virus itself, and to include methods that focus on work
with avian hosts, an area that has been greatly lacking. In that context, this volume
) |# ?% D( X# Y2 ~1 l8 w0 ^6 p( Dwill be of interest to both diagnosticians and researchers, but it does assume a basic
knowledge of virology and molecular biology.
I would like to thank all the authors for their contributions. I am also thankful to
. N9 P0 d4 ^9 E3 m2 G8 a  Q/ ]4 ~John M. Walker, the series editor, for his help in editing and working with me
through this project.
+ ?7 F. k! M' O( V3 ^) ]" WErica Spackman
Athens, GA, September 2007

8 A1 N" b3 l5 j8 b. k【出版日期】：2008
5 q* A! f# C% \' H! k【目    录】：
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
  F6 `3 J9 ?+ {Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
1 A Brief Introduction to the Avian Infl uenza Virus. . . . . . . . . . . . . . . . 1
" r+ r2 ^& v9 Q! m* a- {Erica Spackman
2 Avian Infl uenza Virus Sample Types, Collection, and Handling . . . . . 7
/ D8 l' j+ K, E5 W1 T  }5 kMary Lea Killian
3 Avian Infl uenza Virus RNA Extraction from Tissue
and Swab Material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
' g) N% g2 H: H8 f# k8 Q# KErica Spackman and David L. Suarez
4 Type A Infl uenza Virus Detection and Quantitation
, j9 k/ ~3 e) k" fby Real-Time RT-PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Erica Spackman and David L. Suarez
6 D; z4 d5 d' `$ g2 R/ |5 Detection and Identifi cation of the H5 Hemagglutinin
Subtype by Real-Time RT-PCR. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
* n( h% \& |' s( [; UErica Spackman and David L. Suarez
6 Avian Infl uenza Virus Isolation and Propagation
" g4 [9 D/ Q) h) X7 P# C  _" yin Chicken Eggs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
* r/ T% |2 s% W: X1 `Peter R. Woolcock
7 Hemagglutination Assay for the Avian Infl uenza Virus . . . . . . . . . . . 47
Mary Lea Killian
8 f7 ]* {# ~* ~* D  B$ T; H& P5 H% Q8 Hemagglutination-Inhibition Test for Avian Infl uenza Virus
Subtype Identifi cation and the Detection and Quantitation
of Serum Antibodies to the Avian Infl uenza Virus . . . . . . . . . . . . . . . 53
Janice C. Pedersen
+ G$ h7 I$ i# V$ |+ C: A- V7 }; uvii
9 Neuraminidase-Inhibition Assay for the Identifi cation
& y% S4 }: l3 }4 sof Infl uenza A Virus Neuraminidase Subtype or
3 @0 z$ ^. H2 c$ M: R7 ANeuraminidase Antibody Specifi city . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
9 K6 G5 I9 S& K  UJanice C. Pedersen
10 Immunohistochemical Staining for the Detection of the
Avian Infl uenza Virus in Tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Mary J. Pantin-Jackwood
11 Wild Bird Surveillance for the Avian Infl uenza Virus. . . . . . . . . . . . . 85
Justin D. Brown and David E. Stallknecht
12 Reverse Genetics of the Avian Infl uenza Virus. . . . . . . . . . . . . . . . . . . 99
Chang-Won Lee and David L. Suarez
& T$ a. O$ m7 y0 h13 Evaluating the Cell-Mediated Immune Response of Avian
: `! Q: R- S8 L/ \6 F* q1 x0 \Species to Avian Infl uenza Viruses . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
* H# ^1 }# T/ Y5 i+ J: @5 vDarrell R. Kapczynski
14 Measurement of Avian Cytokines with Real-Time RT-PCR
Following Infection with the Avian Infl uenza Virus . . . . . . . . . . . . . . 127
Darrell R. Kapczynski and Michael H. Kogut
, ?  c6 I; K  G- q$ ]. j0 cIndex . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
viii Contents
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