禽流感病毒Avian Influenza Virus (Methods in Molecular Biology)

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本帖转自 http://biosky.haotui.com/viewthread.php?tid=6402  作者：pyau
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/ q) H9 F. H: O08年新书 Avian Influenza Virus
/ h; X. w  [! t& N, Y【书名版次】：Avian Influenza Virus（First edition）(Methods in Molecular Biology Series 436)  
7 I7 ?. z4 |& U! U  H( r【作    者】：Erica Spackman Editor
【出 版 社】: Humana Press
7 y# e& U4 e/ a0 a# d【简    介】：
   08新书,大家共享    附件PDF下载！
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0 _/ b% [2 u7 a% T   The aim of this book is to create a resource that includes both basic methods that
: M6 c% M1 W: C* x/ Pare currently used and well established as well as some of the most exciting new
" k4 q/ H( |2 }* Lmethods for studying the virus itself, and to include methods that focus on work
6 [) G$ [0 h& k0 h# |with avian hosts, an area that has been greatly lacking. In that context, this volume
will be of interest to both diagnosticians and researchers, but it does assume a basic
5 U- N# _# s, C6 F# A$ ]; P+ Tknowledge of virology and molecular biology.
I would like to thank all the authors for their contributions. I am also thankful to
John M. Walker, the series editor, for his help in editing and working with me
through this project.
+ _) Y+ d9 h2 ]- q* c1 FErica Spackman
Athens, GA, September 2007

) S+ {9 _3 S: z1 y8 o% U【出版日期】：2008
/ P0 R  i& D9 S. q% d5 n* R【目    录】：
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
% T* K" L- m9 kContributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
0 X  Q% k5 q: O# V/ O1 A Brief Introduction to the Avian Infl uenza Virus. . . . . . . . . . . . . . . . 1
Erica Spackman
2 Avian Infl uenza Virus Sample Types, Collection, and Handling . . . . . 7
' I% O2 a0 E2 Z, V4 `) f8 F# \Mary Lea Killian
3 Avian Infl uenza Virus RNA Extraction from Tissue
9 L# x) d9 s8 K1 _and Swab Material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
! L& p, d, B( WErica Spackman and David L. Suarez
4 Type A Infl uenza Virus Detection and Quantitation
- t2 S, D. Q' ^; D% ~6 W0 }by Real-Time RT-PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Erica Spackman and David L. Suarez
5 Detection and Identifi cation of the H5 Hemagglutinin
& a) p, L, q2 e7 G) {" f' k9 T  ^Subtype by Real-Time RT-PCR. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
* ]7 S  U& i3 Y1 Q6 k& GErica Spackman and David L. Suarez
6 Avian Infl uenza Virus Isolation and Propagation
in Chicken Eggs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
3 a$ N: T9 o" PPeter R. Woolcock
7 Hemagglutination Assay for the Avian Infl uenza Virus . . . . . . . . . . . 47
/ k$ |& E0 g0 W' oMary Lea Killian
8 Hemagglutination-Inhibition Test for Avian Infl uenza Virus
3 P: u! w9 {+ ]& ?$ G/ U; T  oSubtype Identifi cation and the Detection and Quantitation
of Serum Antibodies to the Avian Infl uenza Virus . . . . . . . . . . . . . . . 53
Janice C. Pedersen
8 v8 v' q8 @# \' \* S0 b# ^vii
4 Y& s; I( \* }; k' |9 Neuraminidase-Inhibition Assay for the Identifi cation
' h' `( ]- G- bof Infl uenza A Virus Neuraminidase Subtype or
Neuraminidase Antibody Specifi city . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
: [+ d  T+ D3 {5 X8 x) O- xJanice C. Pedersen
10 Immunohistochemical Staining for the Detection of the
Avian Infl uenza Virus in Tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
$ E/ @. ]1 J3 f# S( GMary J. Pantin-Jackwood
11 Wild Bird Surveillance for the Avian Infl uenza Virus. . . . . . . . . . . . . 85
Justin D. Brown and David E. Stallknecht
12 Reverse Genetics of the Avian Infl uenza Virus. . . . . . . . . . . . . . . . . . . 99
Chang-Won Lee and David L. Suarez
: v) i# ~0 b8 w3 u1 h7 q13 Evaluating the Cell-Mediated Immune Response of Avian
Species to Avian Infl uenza Viruses . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Darrell R. Kapczynski
14 Measurement of Avian Cytokines with Real-Time RT-PCR
Following Infection with the Avian Infl uenza Virus . . . . . . . . . . . . . . 127
Darrell R. Kapczynski and Michael H. Kogut
4 x! J% Y1 T" ]" ]1 h, X2 i* ?/ @Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
4 J4 M- B) O4 }% K: ]7 m0 B/ vviii Contents



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