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Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii (Download the PDF)
4 z5 _4 G, l2 t5 w, ~& M. jYing-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*$ c6 N8 b4 \) W, J
& t7 Y( x2 T/ T" BAbstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.
( W/ `$ Q, {, ?& UTherefore, bats may have evolved effective mechanisms to control viral replication. However, little- E) |- ^6 \7 M$ W* Z) y' _
information is available on bat immune responses to viral infection. Type I interferon (IFN) plays a" f3 T2 D F% s; Y, F
key role in controlling viral infections. In this study, we report the cloning, expression, and" f8 m8 c, m; B
biological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We
5 f: M$ [, Q! \& H% h1 cdemonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from3 p& r5 e. |( s J* t% L7 y* l
M. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant
6 j' L+ N% j9 A" k3 w# C/ HIFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat6 S8 G- U: W6 @+ j7 |) V9 i
species, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral
+ z& h) ^1 _: A, hactivity in microbats, which has important implications for virus interactions with these hosts.
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" Z7 i6 l4 `2 _Keywords: bat; interferon; IFN-stimulated genes; antiviral activity( m4 b8 r( e" g7 X* v& l# V
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中文信息：
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大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测
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梁英子，吴利军，张倩，周鹏，王媚娘，杨兴娄，葛行义，王林发，石正丽*
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4 x' j4 S2 g6 I8 ?: F摘要：蝙蝠是多种病毒的自然宿主，其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状，其中机制未知。近期对两种蝙蝠基因组序列分析结果提示，蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上，用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平；表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白，测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用，试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。
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- T3 ~- h- q' N6 u. L0 M8 a关键词：干扰素，蝙蝠天然免疫，抗病毒活性，定量PCR2 s1 O$ f6 x3 G( s2 u
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发表于 2015-12-7 17:01:33
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发表于 2015-12-7 22:28:08
