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Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii (Download the PDF)$ P, e; ^ C Z: S; Y* B
Ying-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*
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Abstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.9 O) |8 w9 H0 h! g9 ^- d
Therefore, bats may have evolved effective mechanisms to control viral replication. However, little
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key role in controlling viral infections. In this study, we report the cloning, expression, and% S) I8 Q6 P' o4 B3 `1 h
biological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We0 s$ j3 Z/ H* }8 |! _5 X! b
demonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from) C3 z0 _3 U% Q" s- ~0 c
M. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant3 d2 F5 y3 A* k9 T& m' O2 U2 D Y& ~: Z
IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat; u" B5 P0 I0 |- T) U
species, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral: C; S) B+ Q' s- v
activity in microbats, which has important implications for virus interactions with these hosts.: l! [$ z4 o, H0 d8 V
, W1 x T% L7 L, t4 ]Keywords: bat; interferon; IFN-stimulated genes; antiviral activity2 Y) ~: `! X9 ] q2 g2 s
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中文信息：
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大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测2 l5 q2 O% P8 T( e7 N
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梁英子，吴利军，张倩，周鹏，王媚娘，杨兴娄，葛行义，王林发，石正丽*
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摘要：蝙蝠是多种病毒的自然宿主，其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状，其中机制未知。近期对两种蝙蝠基因组序列分析结果提示，蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上，用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平；表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白，测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用，试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。
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关键词：干扰素，蝙蝠天然免疫，抗病毒活性，定量PCR
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发表于 2015-12-7 17:01:33
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发表于 2015-12-7 22:28:08
