免疫试验手册（英文版）

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% q) P) k6 l, d/ p5 `) i: l3 O! `, {      免疫试验手册（英文版）


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IMMUNOLOGY
60.401
0 t- L$ l  R& }/ }& {- VLAB MANUAL 2004
( O4 i. f- c, `- f  U5 t不错的书，下面是目录
2 p# R! _1 L8 s5 oSCHEDULE 2
GENERAL INSTRUCTIONS 4
4 w  ]! _  `0 c; L) ?! S* OREFERENCE BINDER CONTENTS 8
* q% V4 W7 ~) `2 o& VSTANDARD OPERATIONS PROCEDURE 9
WHMIS 11
) D0 Q$ Y# X/ B' S% n/ RLab 1: Preparation of Crude IgG by Salt Fractionation 13
Lab 2: Immunoprecipitation 17
0 y+ J* E* `4 H& MLab 3: Hemagglutination Assay 28
Lab 4: Introduction to the Enzyme Linked Immunosorbent Assay (ELISA) 33
# R  O& h3 Q* X, X  e/ I. g8 JLab 5: Part I: Induction and characterization of a primary cytokine 38
response in vivo.
Part II: ELISA precision training.
9 J- H( }) Z& t% I/ YLab 5: Part III: ELISA ASSAY- Quantitative analysis of IL-13 Response 47
Appendix:
/ S& `; F( g8 s/ A' iSolutions 53
# P, O1 w6 W2 f2 L/ uFloor Model Centrifugation Operation 54
Pipetman Operation 56
$ e3 P0 C: {/ w( W( MElectrophoresis 58
Sample Lab Exam 60