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本帖最后由 序道 于 2015-2-3 19:21 编辑 , l6 l; T: p6 N9 b0 K
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IMMUNOLOGY& q7 [' Z) t0 B$ ]+ p+ ^
60.401: A* s. U5 H. i
LAB MANUAL 2004
: ~# o1 ? {+ e不错的书,下面是目录 . H+ j, _' g; X3 g/ ~/ p. V
SCHEDULE 24 P+ |& U4 G4 b- W- Y9 z
GENERAL INSTRUCTIONS 4 7 e2 j7 Q3 ^0 u& k
REFERENCE BINDER CONTENTS 8
0 ?& [, m2 s! e' k8 \( ZSTANDARD OPERATIONS PROCEDURE 9
( c' k2 Y7 j8 f6 GWHMIS 11' T- A1 y* d; z
Lab 1: Preparation of Crude IgG by Salt Fractionation 13
$ L7 e2 [3 G$ a5 r( {% YLab 2: Immunoprecipitation 17
4 v5 Q: b6 H! d' J" J" GLab 3: Hemagglutination Assay 28
% D! f8 r6 W( A; qLab 4: Introduction to the Enzyme Linked Immunosorbent Assay (ELISA) 33
+ M4 }; Z; I) W. i( L+ aLab 5: Part I: Induction and characterization of a primary cytokine 38
$ f, V2 M* Z$ M! u% Oresponse in vivo.7 w' |0 i% G/ O( s' W( Z
Part II: ELISA precision training.
; C7 e+ V$ G+ J7 @Lab 5: Part III: ELISA ASSAY- Quantitative analysis of IL-13 Response 47
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Solutions 53 s- G& I. _7 Y! g. p# n
Floor Model Centrifugation Operation 54
% z8 M/ y) V$ g0 qPipetman Operation 56! j0 |! B' ^ R5 z% X0 Z/ f' ~9 s. `
Electrophoresis 589 g# {) }- i+ s7 X
Sample Lab Exam 60 |
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