ATCC官方——动物细胞培养手册（Animal Cell Culture Guide）

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      动物细胞培养手册有助于我们做病毒研究的人，及时了解细胞特性及更好的运用细胞来为我们科研服务。

下面为ATCC官方的一些培养手册目录如下：


Getting Started with an ATCC Cell Line.............1
Product Sheet..............................................................1
Preparation of Medium............................................1
Initiating Frozen Cultures........................................1
Processing Flask Cultures........................................1
Cell Growth and Propagation....................................4
Passage Number and Population Doubling
Level................................................................................4
Adapting to a New Medium or Serum...............5
Temperature.................................................................5
Examination of Cultures..........................................6
Cell Counting...............................................................6
Cell Viability..................................................................7
Subculturing Monolayer Cells...............................8
Monolayer Subculturing..........................................8
Troubleshooting Monolayer Cell Subculturing...9
Suspension Cells......................................................10
Suspension Cell Subculturing.............................10
Troubleshooting Suspension Cell Subculturing...11
Adapting a Monolayer Cell Line to Grow in
Suspension................................................................11
Complete Growth Media.............................................13
Cell Culture Media...................................................13
Media Formulations...............................................14
Media Ingredients...................................................14
Media Supplements...............................................16
Osmolality..................................................................17
Antibiotics and Antimycotics..............................17
Animal Sera...............................................................18
Culture Vessels and Surfaces...................................20
Vessels.........................................................................20
Surface Coatings and Feeder Cells....................22
Cryopreservation...............................................................24
Overview....................................................................24
Freeze Medium........................................................24
Equipment.................................................................25
Liquid Nitrogen Freezer Storage.......................26
Cryopreservation Procedure...............................26
Recovery of Cryopreserved Cells.......................27
Contamination and Biosafety.................................28
Check for Microbial Contamination.................28
Mycoplasma Contamination...............................28
Treating for Microbiobial Contamination.......29
Cellular Cross-Contamination.............................29
Biosafety.....................................................................30
Glossary......................................................................................31
References................................................................................34
ATCC Media, Sera, and Reagents.........................35

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Biodog 发表于 2015-4-20 21:28
感谢版主的分享！

客气了啊 共同学习

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hydwangyong 发表于 2015-4-21 08:14
谢谢分享

客气了  共同学习进步

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cmo 发表于 2015-4-22 11:02
感谢分享

客气了啊

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xiaogang 发表于 2016-8-13 08:33
谢谢

客气啦，共同学习

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hantavirus 发表于 2016-8-17 13:17
序道版主您好，您是潍坊的吗？

我不是潍坊的，不过我短暂的在潍坊工作

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virus_cell 发表于 2016-8-17 17:48
隐藏的内容哪里看得到？

回复就能看到啊

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xiaogang 发表于 2017-5-24 11:52
貌似回复了也没看到下载内容

电脑版本可见  客户端看不见

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rapido 发表于 2017-7-28 13:59
好参考资料啊，谢谢分享