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Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii (Download the PDF)
( K! t( E+ U* r+ w2 ]# a* m, uYing-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*" {, \9 k. k7 s9 r0 y# T
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Abstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.7 M9 ]4 t/ l9 s
Therefore, bats may have evolved effective mechanisms to control viral replication. However, little0 U# ]% b7 _/ S, {9 S: x6 c
information is available on bat immune responses to viral infection. Type I interferon (IFN) plays a) h! I. u9 S' U9 \( v
key role in controlling viral infections. In this study, we report the cloning, expression, and& |, k; l& q* D/ T0 }7 l) w
biological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We
, g4 a; n: s/ o* s9 b( D8 a5 ~; n/ Kdemonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from
# W7 \/ W7 z. `M. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant, O" S% \# d1 m4 |
IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat
2 [5 ~ q# V+ k r& nspecies, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral
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Keywords: bat; interferon; IFN-stimulated genes; antiviral activity J' q7 W: P+ F. m6 B2 Y5 l: F
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大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测
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梁英子,吴利军,张倩,周鹏,王媚娘,杨兴娄,葛行义,王林发,石正丽*
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摘要:蝙蝠是多种病毒的自然宿主,其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状,其中机制未知。近期对两种蝙蝠基因组序列分析结果提示,蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上,用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平;表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白,测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用,试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。
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% I9 y h7 j' \+ w关键词:干扰素,蝙蝠天然免疫,抗病毒活性,定量PCR V- _/ e! d/ t' _+ R B. t
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