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Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii           (Download the PDF)
2 ^1 x) ^7 q) k$ w2 v& eYing-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*

0 g, o; f& E  H1 D; ~" lAbstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.
Therefore, bats may have evolved effective mechanisms to control viral replication. However, little
. u2 t; z9 A5 D( q- C( }3 c& Q; cinformation is available on bat immune responses to viral infection. Type I interferon (IFN) plays a
1 v% P( V' F5 ]2 Z) `6 v7 bkey role in controlling viral infections. In this study, we report the cloning, expression, and
7 @( L3 ?2 n; x# p( _biological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We
1 {5 W5 Y2 O9 W9 F# odemonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from
M. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant
IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat
7 @4 q, p$ |7 J6 }* h& e+ H0 v# Q- l! [species, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral
" I: R" l" L6 E) yactivity in microbats, which has important implications for virus interactions with these hosts.

Keywords: bat; interferon; IFN-stimulated genes; antiviral activity

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! T1 _  v! ?9 T2 n# |中文信息：

大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测


1 }3 Y8 _5 m' ^. i, q% c! U梁英子，吴利军，张倩，周鹏，王媚娘，杨兴娄，葛行义，王林发，石正丽*
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摘要：蝙蝠是多种病毒的自然宿主，其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状，其中机制未知。近期对两种蝙蝠基因组序列分析结果提示，蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上，用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平；表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白，测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用，试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。
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. e* G8 E. a2 G; e! i. C关键词：干扰素，蝙蝠天然免疫，抗病毒活性，定量PCR
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