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Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii           (Download the PDF)
Ying-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*
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! X. @' [2 h7 @% ?/ H1 CAbstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.
Therefore, bats may have evolved effective mechanisms to control viral replication. However, little
$ N5 b3 S. ?6 X+ p; e; d  minformation is available on bat immune responses to viral infection. Type I interferon (IFN) plays a
. W$ T$ r# T3 l3 G4 S* o# okey role in controlling viral infections. In this study, we report the cloning, expression, and
biological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We
  t) u8 F1 N' A5 v/ S9 q: ?demonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from
M. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant
9 h* F: L1 D# H* {' v! {IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat
: m6 d) u; k2 f. E% B7 m# U, g; uspecies, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral
activity in microbats, which has important implications for virus interactions with these hosts.
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Keywords: bat; interferon; IFN-stimulated genes; antiviral activity

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大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测

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8 B7 R2 a6 o+ L梁英子，吴利军，张倩，周鹏，王媚娘，杨兴娄，葛行义，王林发，石正丽*

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摘要：蝙蝠是多种病毒的自然宿主，其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状，其中机制未知。近期对两种蝙蝠基因组序列分析结果提示，蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上，用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平；表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白，测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用，试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。


, Y; j; c$ s; q# ~3 m! j3 Z9 r关键词：干扰素，蝙蝠天然免疫，抗病毒活性，定量PCR
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