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Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii           (Download the PDF)
Ying-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi*
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7 ~2 U) O% K2 aAbstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.
7 ]- `3 s6 T* T8 f( |& l& ZTherefore, bats may have evolved effective mechanisms to control viral replication. However, little
% c; u  h% L& n. \6 x3 pinformation is available on bat immune responses to viral infection. Type I interferon (IFN) plays a
key role in controlling viral infections. In this study, we report the cloning, expression, and
biological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We
demonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from
M. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant
, ~5 x- W0 g; vIFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat
# d8 G. _6 A- ~8 I+ J* @) H0 D* cspecies, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral
0 r2 I1 q# k- E! W! e  jactivity in microbats, which has important implications for virus interactions with these hosts.

, d1 ~( D! B* u9 S/ f4 ~. VKeywords: bat; interferon; IFN-stimulated genes; antiviral activity
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中文信息：
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1 g( E, J, I( i% ]4 g大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测


! S$ a: D+ M& J/ a; k4 b$ H梁英子，吴利军，张倩，周鹏，王媚娘，杨兴娄，葛行义，王林发，石正丽*


4 s9 G2 u7 W$ M- a+ t) ?摘要：蝙蝠是多种病毒的自然宿主，其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状，其中机制未知。近期对两种蝙蝠基因组序列分析结果提示，蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上，用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平；表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白，测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用，试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。


关键词：干扰素，蝙蝠天然免疫，抗病毒活性，定量PCR

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