标题: Virologica Sinica 精彩论文抢先看！ [打印本页] 作者: lishaojie 时间: 2015-12-7 17:01 标题: Virologica Sinica 精彩论文抢先看！ Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii (Download the PDF)1 }$ q6 Z E8 Y3 o Ying-Zi Liang, Li-Jun Wu, Qian Zhang, Peng Zhou, Mei-Niang Wang, Xing-Lou Yang, Xing-Yi Ge, Lin-Fa Wang, Zheng-Li Shi* 2 @0 A/ Y1 q% Q( G# e b8 |# ^" H% M) I Abstract: Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.. a5 x8 f: C( [& d' e* D- u
Therefore, bats may have evolved effective mechanisms to control viral replication. However, little9 W I6 e d+ w& D5 ^5 { b; ]) S
information is available on bat immune responses to viral infection. Type I interferon (IFN) plays a u0 j4 e$ e6 y4 k9 n
key role in controlling viral infections. In this study, we report the cloning, expression, and 8 H# z' p$ `2 n) P- M* Hbiological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We, O1 @$ I1 B4 e/ `
demonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from : R$ O7 R! J: Q: I% RM. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant# u- s7 Y" [ F0 A5 ~: I: u2 C: f" U; U
IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat: Q, c1 i, S. h/ S N
species, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral* M/ k9 Z- B) P9 B1 G7 `
activity in microbats, which has important implications for virus interactions with these hosts. . \3 g$ L- h' R & ?+ ^9 j9 d: y) q7 I% g' o. qKeywords: bat; interferon; IFN-stimulated genes; antiviral activity$ J9 A" [2 B6 {6 ~7 O K5 Q
0 s( S4 e; R; v, f, c' I4 D( l/ }1 |- K& ?6 `4 t4 t& w 中文信息： . ?7 Y$ \; i1 ~. l0 y# Y, `8 d- t. i" G, C 大卫鼠耳蝠干扰素β的克隆、表达和抗病毒活性检测1 e4 @* B5 v% c, s ) G' M5 [. y: v4 q, w4 o! ~; ?9 a* z$ s) o& T# B: C0 `) U& Y
梁英子，吴利军，张倩，周鹏，王媚娘，杨兴娄，葛行义，王林发，石正丽*5 n: C6 T( v. c, n- q3 S
& V' H: a* p# I. f8 @. b& f 5 N6 m p- {5 v' W6 b5 p摘要：蝙蝠是多种病毒的自然宿主，其中一些病毒是人畜新发传染病的病原。然而自然携带或人工感染病毒的蝙蝠并不表现出临床症状，其中机制未知。近期对两种蝙蝠基因组序列分析结果提示，蝙蝠可能具有一些独特的先天性免疫机制。本论文在前期研究基础上，用定量PCR技术分析病毒感染后蝙蝠干扰素及其诱导基因的应答水平；表达鼠耳蝠Ⅰ型干扰素和干扰素诱导蛋白，测试蝙蝠干扰素在不同来源的细胞中对病毒的抑制作用，试图初步了解病毒诱导的蝙蝠先天性免疫应答机制。 1 N& H, A& ?' R# t0 ]4 G1 M; L' y 1 u+ i# l3 E; d5 e: X0 b! p" x4 c z+ e% H9 o 关键词：干扰素，蝙蝠天然免疫，抗病毒活性，定量PCR ; |8 W5 u. @. L3 E' U$ ~4 k. m9 j% z9 ?* W5 N+ ~$ \+ C+ s i8 r& N 作者: rojjer 时间: 2015-12-7 22:28
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